Sr. Research Specialist
Johns Hopkins University

Baltimore, Maryland

Posted in Education and Training

This job has expired.

Job Info

Sr. Research Specialist
The Institute for Cell Engineering is seeking a highly motivated individual with interest and expertise in cell biology and molecular biology to work as a Senior Research Specialist. This role will participate in ongoing projects involving the study of pathogenesis of neurodegenerative diseases utilizing human induced pluripotent stem cell (iPSC)-derived neural cells.

Under the direction of lab Principle Investigator (PI), this individual will assist the Senior Research Fellow in the implementation of differentiation of human pluripotent stem cell lines, engineering of stems cells and neural tissue-like structures/organoids to model Stroke, Alzheimer's disease (AD) and AD related dementia (ADRD). This position plays an important role at Johns Hopkins School of Medicine by enabling investigators to perform state-of-the-art cellular, molecular and/or developmental biology experimentation in patient pluripotent stem cells and derived cells.

Specific Duties & Responsibilities:

Conduct Research Under the Direction of Senior Research Fellow:

  • Basic responsibilities will include, but not be limited to, all aspects of cell culture, including media, reagent, buffer ordering and preparation, culture maintenance, cryopreservation of cells and work with both primary and stem cell lines. Executes basic molecular biology: PCR, molecular cloning, mini/maxi prep, Western blot, histochemistry and immunocytochemistry using fluorescence microscopy and Confocal, etc.
  • Perform neuronal differentiation of multiple neuronal subtypes and phenotyping, including high content/high throughput image analysis, multielectrode array recordings for functional analysis. Analyze stem cell cultures and their derivatives using wide range of assays including ELISA, q-PCR, single cell RNA seq and flow cytometry.
  • Investigate mechanisms that underly the unique properties of each neuron subtype to meet the demands of multiple concurrent projects.
  • Creation and maintain iPSC-derived organoids.
  • Helps develop and carryout new techniques for various studies ongoing in the lab. Perform other various laboratory tasks as requested.
  • Provides training/ supervision to other individuals who may be assigned to the laboratory on a regular or temporary basis.
  • In collaboration with PIs, will make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction.
  • Meets regularly with the PI to discuss study progress.
  • Assist in the completion of ACUC and IRB applications
  • Performs miscellaneous laboratory duties as assigned.

Minimum Qualifications (Mandatory):
  • Bachelor's Degree in Biological Sciences or related field plus four years in a comparable laboratory performing relevant techniques.
  • Master's Degree, with related graduate research, may substitute for experience to the extent permitted by the JHU equivalency formula.*

* JHU Equivalency Formula: 30 undergraduate degree credits (semester hours) or 18 graduate degree credits may substitute for one year of experience. Additional related experience may substitute for the required education on the same basis. For jobs where equivalency is permitted, up to two years of non-related college course work may be applied towards the total minimum education/experience required for the respective job.

** Applicants who do not meet the posted requirements but are completing their final academic semester/quarter will be considered eligible for employment and may be asked to provide additional information confirming their academic completion date.

Special Knowledge, Skills & Abilities:
  • Excellent organizational skills, ability to exercise professional judgment and good written and oral communication skills is required.
  • The position may require flexible working hours to meet demands for maintenance of cell culture experiments based on projects assigned.

Preferred Qualifications:
  • Research background and knowledge of Neuroscience, Development and/or Cell Biology.
  • Working experience with cell culture, ideally human embryonic or induced pluripotent stem cells (iPS).
  • Scientific background in disease modeling and/or the use of human iPS and their derivatives to model human disease.
  • Quick-learner and desire to learn novel stem cell and neuroscience techniques.

Classified Title: Sr. Research Specialist
Role/Level/Range: ACRP/04/MC
Starting Salary Range: $45,650 - $62,850 annually (commensurate with experience)
Employee group: Full Time
Schedule: M - F, 8:30 - 5:00
Exempt Status: Exempt
Location: School of Medicine Campus
Department name: SOM ICE Neuroregeneration
Personnel area: School of Medicine

The successful candidate(s) for this position will be subject to a pre-employment background check.

If you are interested in applying for employment with The Johns Hopkins University and require special assistance or accommodation during any part of the pre-employment process, please contact the HR Business Services Office at For TTY users, call via Maryland Relay or dial 711.

Johns Hopkins has mandated COVID-19 and influenza vaccines, as applicable. Exceptions to the COVID and flu vaccine requirements may be provided to individuals for religious beliefs or medical reasons. Requests for an exception must be submitted to the JHU vaccination registry. For additional information, applicants for SOM positions should visit and all other JHU applicants should visit

The following additional provisions may apply, depending on campus. Your recruiter will advise accordingly.

The pre-employment physical for positions in clinical areas, laboratories, working with research subjects, or involving community contact requires documentation of immune status against Rubella (German measles), Rubeola (Measles), Mumps, Varicella (chickenpox), Hepatitis B and documentation of having received the Tdap (Tetanus, diphtheria, pertussis) vaccination. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing. Blood tests for immunities to these diseases are ordinarily included in the pre-employment physical exam except for those employees who provide results of blood tests or immunization documentation from their own health care providers. Any vaccinations required for these diseases will be given at no cost in our Occupational Health office.

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