Position OverviewThe Unckless Lab in the Department of Molecular Biosciences, University of Kansas, seeks an assistant researcher with interests and experience in molecular biology, invertebrate immunity and virology. The successful candidate will work with a research group that addresses the broad themes of host-pathogen interaction and genetic conflict. Specifically, the assistant researcher would assist with an NSF-funded study of the genetics of a large DNA virus. The work would involve cloning the virus into a bacterial artificial chromosome (BAC), performing genetic manipulations of the virus, and optimizing cell culture conditions for optimal viral replication. Work will include cell culture, molecular cloning, viral genome sequencing, CRISPR/Cas9 genome editing and microscopy. The position consists of an initial 2-yr appointment beginning in fall 2021. The applicant should be extremely organized, work well with others, have a willingness to learn, and be able to participate at many levels in the laboratory. Job Description50% Cloning a DNA virus into a bacterial artificial chromosome (BAC). Experiments to isolate high molecular weight viral DNA, manipulate plasmid vectors, clone the virus and screen for recombinants. Eventually, this would include genetic manipulation of the virus. These experiments require considerable molecular biology and cell culture work.
20% Optimizing conditions for viral growth in cell culture. Develop new cell lines from relevant species and tissues. Experiment with different media, temperatures, nutrients, etc. and perform assays to measure viral replication and growth.
20% Work on an independent research project. If interested, the assistant researcher can initiate their own research project related to the theme of host/virus interaction if agreed upon with the PI.
10% Experiments in live Drosophila. Infect Drosophila with the DNA virus and measure mortality, viral titer, host gene expression, etc.